Journal:

Article Title: Axoplasm Isolation from Peripheral Nerve

doi: 10.1002/dneu.20755

Figure Lengend Snippet: (A) Electron micrograph of negative staining of axoplasm from the isotonic squeeze method shows a nonhomogeous preparation containing vesicular structures of different sizes. (B) Negative staining of axoplasm from the hypotonic extraction procedure shows a more homogenous preparation containing numerous small size vesicle-like structures. Scale bar in both images 200 nm. (CF) Western blot comparison of soluble and pellet fractions of axoplasms extracted by different methods and cleared by regular centrifugation or ultracentrifuge (UC). Albumin and transferrin were used to monitor serum contamination, CNPase and GFAP for Schwann cells and other glia, importins and dynein intermediate chain (IC) for cell body components and retrograde signaling complexes, and general Erk1 and Erk2 MAP kinases (gERK) as a loading control. (G) Levels of different proteins in the soluble fraction from hypotonic extraction axoplasm as a percentage of their level in isotonic squeeze axoplasm. All data was normalized to the average of the control group (isotonic squeeze axoplasm), taken as 100% in each blot. Statistics were by one-sample t-test with hypothesized population mean 100, p-value < 0.05. Note markedly reduced levels of serum and glia contaminants, versus enrichment of dynein associated proteins. 80 μg protein per lane.

Article Snippet: Mouse anti-Dynein intermediate chain clone 74.1 was from Chemicon (MAB1618), rabbit anti-NFH was from Chemicon (AB1989); mouse anti-NFH clone N52 was from Sigma; mouse anti-Importin β clone 31H4 was from Sigma (I2534); mouse anti-CNPase was from Chemicon (MAB326); mouse anti-GFAP clone G-A-5 was from Sigma (G6171); rabbit anti-albumin and rabbit anti-transferrin were from Cedarlane (CLAG5140 and GLAG5240 respectively); mouse anti-tubulin β3 was from Sigma (T2200); and rabbit anti-gERK was from Sigma (M5670).

Techniques: Negative Staining, Western Blot, Centrifugation

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: RT-qPCR analysis of DNMTs and IL1R2 expression, along with IHC staining (A) RT-qPCR was used to quantitatively analyze the expression profiles of DNMTs and IL1R2 in HBW and LBW placentas ( DNMT3A : p = 0.0264; DNMT3L : p = 0.0016; DNMT1 : p = 0.0069; IL1R2 : p = 0.0156). (B and D) IHC was performed to analyze the protein expression intensity of DNMT3A in HBW and LBW placentas (DNMT3A: p = 0.0059). Scale bars, 50 μm. (C and E) IHC was performed to analyze the protein expression intensity of IL1R2 in HBW and LBW placentas (IL1R2: p = 0.0007). Scale bars, 50 μm. Data are represented as mean ± SEM. Statistical significance was assessed by paired Student’s t test, with n = 6 (3 samples in the LBW group and 3 samples in the HBW group).

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Quantitative RT-PCR, Expressing, Immunohistochemistry

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: MethPrimer-based prediction of CpG islands in the IL1R2 promoter region and subsequent analysis of the methylation status in placental tissues (A) Bioinformatics analysis predicted CpG islands in the promoter region of IL1R2 . (B) MS-PCR measured IL1R2 methylation levels in HBW and LBW placentas ( p = 0.0062). Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, with n = 6 (3 samples in the LBW group and 3 samples in the HBW group).

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Methylation

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: Effects of 5-Aza treatment on IL1R2 gene expression and methylation levels in PTr2 cells (A) The mRNA expression of IL1R2 in 5-Aza-treated PTr2 cells was measured using RT-qPCR (20 μM: p = 0.0199). “ns” denotes non-significant results ( p > 0.05). (B) MS-PCR measured the methylation levels of the IL1R2 promoter in 5-Aza-treated PTr2 Cells ( p = 0.0005). (C) BS-PCR measured the methylation levels of the IL1R2 promoter in 5-Aza-treated PTr2 cells ( p = 0.0011). Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Gene Expression, Methylation, Expressing, Quantitative RT-PCR

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: IL1R2 knockdown promotes PTr2 Cell proliferation and migration (A) RT-qPCR analysis of IL1R2 knockdown efficiency and its effect on proliferation and apoptosis markers in PTr2 cells ( IL1R2 : p = 0.0002; Ki67 : p = 0.0043; BAX : p = 0.0155; CASP3 : p = 0.0088; CASP9 : p = 0.0331). “ns” denotes non-significant results ( p > 0.05). (B) Cell proliferation assessed using CCK-8 assay after IL1R2 knockdown (48 h: p = 0.0224; 72 h: p = 0.0035). (C and D) Cell proliferation evaluated using EdU assay ( p = 0.0083). Scale bars, 100 μm. (E and F) Cell migration was analyzed by scratch assay ( p = 0.0462). Scale bars, 500 μm. Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Knockdown, Migration, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Wound Healing Assay

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: IL1R2 overexpression inhibits PTr2 Cell proliferation and migration (A) RT-qPCR analysis of IL1R2 overexpression efficiency and its effect on proliferation and apoptosis markers in PTr2 cells ( IL1R2 : p = 0.0003; PCNA : p = 0.0256; BAX : p = 0.0497). “ns” denotes non-significant results ( p > 0.05). (B) Cell proliferation was assessed using CCK-8 assay following IL1R2 overexpression (48 h: p = 0.0027; 72 h: p = 0.0011). (C and D) Cell proliferation evaluated using EdU assay ( p = 0.0452). Scale bars, 100 μm. (E and F) Cell migration was analyzed by scratch assay ( p = 0.0078). Scale bars, 500 μm. Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Over Expression, Migration, Quantitative RT-PCR, CCK-8 Assay, EdU Assay, Wound Healing Assay

Journal: iScience

Article Title: DNA methylation of the IL1R2 gene is associated with porcine placental development and birth weight

doi: 10.1016/j.isci.2026.115055

Figure Lengend Snippet: IL1R2 positively regulates TNF- α expression in PTr2 cells (A) IL1R2 knockdown suppresses TNF-α mRNA levels ( p = 0.0442). (B) IL1R2 overexpression elevates TNF-α mRNA levels ( p = 0.0057). (C - E) IL1R2 knockdown reduces TNF- α protein abundance, with confirmation of knockdown efficiency (IL1R2: p = 0.0012; TNF-α: p = 0.0394). (F - H) IL1R2 overexpression increases TNF- α protein levels, with confirmation of knockdown efficiency (IL1R2: p = 0.0011; TNF-α: p = 0.0014). Data are represented as mean ± SEM. Statistical significance was assessed by unpaired Student’s t test, n = 3.

Article Snippet: Rabbit anti-Homo sapiens (Human) IL1R2 Polyclonal antibody , Cusabio Biotech , Cat# CSB-PA011622ESR1HU; RRID: AB_3719906.

Techniques: Expressing, Knockdown, Over Expression, Quantitative Proteomics